As part of my current DENOCS project I am affiliated with the Alfred Wegener Institute in Bremerhaven. I am working with Prof. Doris Abele and Dr. Julia Strahl on the visualisation of Nitric Oxide in an intact coral symbiosis. To do this I have been adopting and modifying existing protocols to create coral tissue cultures.
Coral tissue cultures are consisting of organised coral host – algal symbiont associated cells but do not contain a skeleton. The huge advantage is that these tissue cultures can then be used for confocal microscopy, where with the help of lasers a three-dimensional imaging assay can then be applied. In the current project we applied specific fluorescent dyes which allow localization of key radicals. With the right experimental design this technique can help answering a lot of questions about radical localization, temporal dynamics in radical production and specific type of radical formation in dependence of the acting stressor. Stay tuned for upcoming publications and updates on our findings.